Winter Run

[Observer]

ok sorry im unloading research now


you got me back in it

1. Heat Stress and Enzyme Activity​

Temperature Effects on Enzymes:

• Enzyme Denaturation: High temperatures can cause denaturation of enzymes involved in various metabolic processes. Enzymes such as those responsible for protein synthesis, hormone regulation, and secondary metabolite production may lose their functional shape and efficiency, disrupting normal plant growth and development.
• Reduced Enzyme Activity: Elevated temperatures can reduce the activity of critical enzymes like ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), which is essential for photosynthesis. This can lead to reduced photosynthetic rates and energy production, affecting overall plant health and growth.

2. Hormonal Imbalances​

Auxins and Gibberellins:

• Auxins: These are plant hormones that regulate cell elongation and differentiation. Heat stress can alter auxin distribution and signaling, leading to abnormal growth patterns and foxtailing.
• Gibberellins: These hormones promote cell elongation and bud development. High temperatures can cause an imbalance in gibberellin levels, leading to excessive elongation of the internodes and abnormal bud formation.

3. Photosynthesis and Respiratory Changes​

Photosynthetic Efficiency:

• Photosynthetic Rate: High temperatures can decrease the efficiency of the photosynthetic process, reducing the production of carbohydrates and other essential compounds needed for healthy bud development.
• Respiration Increase: Heat stress can increase the rate of respiration, leading to higher energy consumption and reduced availability of energy for growth and development.

4. Heat Shock Proteins​

Role of Heat Shock Proteins:

• Protein Production: In response to high temperatures, plants produce heat shock proteins (HSPs) to protect other proteins from damage. HSPs assist in refolding denatured proteins and help maintain cellular function under stress.
• Stress Response: The production of HSPs is a protective response that can alter normal growth patterns and development, potentially leading to abnormal bud and calyx formation.

5. Thermal Regulation and Plant Structure​

Stomatal Closure:

• Water Loss: High temperatures can cause the stomata (tiny openings on leaves) to close to reduce water loss. This can limit gas exchange, affecting CO2 availability and photosynthesis.
• Heat Stress Impact: Stomatal closure can exacerbate heat stress by limiting the plant’s ability to cool itself and regulate internal temperatures.

Cellular Structure:

• Cell Membrane Integrity: Heat stress can disrupt the integrity of cellular membranes, causing leakage of cellular contents and affecting cell function. This can lead to abnormal growth patterns and structural changes in buds and flowers.

6. Reproductive Response​

Calyx Stacking:

• Stress Response: In response to heat stress, plants may increase the density of calyxes as a defensive mechanism. This can be a way to protect the reproductive parts and ensure successful pollination despite adverse conditions.
• Altered Flowering Patterns: High temperatures can alter the flowering cycle, leading to changes in the development and arrangement of calyxes.

7. Gene Expression​

Heat Stress Genes:

• Heat-Responsive Genes: High temperatures trigger the expression of heat-responsive genes that are involved in stress responses and adaptation. These genes can influence various physiological processes, including growth, development, and reproductive structures.
• Transcription Factors: Heat stress can affect the activity of transcription factors that regulate gene expression, leading to changes in growth patterns and reproductive structures.

 

[Observer]

theres a lot of

if it does this, you can do this

if this happens you can try this

if you cant do this one thing, you can do this other thing though.


lol

 

[Observer]

Yeah and with the eventual move to rockwool slabs. That's likely even more into the future.

Since we move the trop cherry really well it likely be trial and error with the drip system on a smaller scale outside of the main grow.

im working on something that may even perform better than rockwool, but i gotta experiment with it first, after getting it printed.

 

[CookiesLikeWhoa]

was trying to see what youre nutrient inputs were, "organics" or salts?

Running Jacks /w PowerSi and Purpinator. PowerSi is out since we are into week 6.

 

[Observer]

Running Jacks /w PowerSi and Purpinator. PowerSi is out since we are into week 6.

ahh right, now i remember.

 

[CookiesLikeWhoa]

Alright almost another week in the books. Trop Cherry are at 48F and the Dante's are at 53F. It's been cooler which has been a blessing. Tents are only getting into the mid 80's now. TCs are just packing on weight. Dante's terps are coming in so strong its creeping out of the garage. Smells like grape soda, like a lot.

Trop Cherry: With three weeks to go, things are looking good. The lighter tent is also the hotter tent in the back of the Garage. They were also the CO2 tent so they've always been kinda different. Both tents are chugging along nicely and packing on a lot of weight.

View attachment IMG_8624.mov

Dante's Inferno: It's a hot mess, but god damn do they smell amazing. Our neighbor asked if we started making grape soda it smelled so strongly. Can't complain about that.


Overall things are going good, temps are down and the plants have been thriving.

Temp: 82F
RH%: 57
PPM: 1020
PPFD: 850

 

[CookiesLikeWhoa]

Another week in the books.

Dante’s at 59F and TC at 54F and couldn't be happier. It's been interesting watching these two different tents of TC and how different they are. The Dante's inferno has also been fun to say the least. Excited to do a proper run of it in the future. There are a few monsters lurking in that tent, but a lot of it is going to end up being larf.

Trop Cherry: Lawdy these girls are getting fat. Difference in the two tents is wild. The CO2 tent, lighter one, used to be far ahead, but since cutting out the CO2 it's fallen behind. We think the with it being like 4 degrees hotter in the CO2 tent caused some issues, but they're still looking great.


Dante's Inferno: It's over grown, a mess, but there are some monsters lurking in the weeds. Managed to capture a few shots. Filling out nicely and the terps are crazy.


Temp: 80F
RH%: 57
PPM: 1020
PPFD: 850

 

[brettdawg]

Looking good stacking nicrly. What co2 you using tanks or bags that you can hang.

 

[TomH]

Gorgeous ladies you hang with there!

 

[CookiesLikeWhoa]

Looking good stacking nicrly. What co2 you using tanks or bags that you can hang.

CO2 tanks, 25lb ones. Tank usually lasted 2-3 weeks @1200PPM. Flow was .5 cubic feet/min with it on.

When we were running it, that tent was packing on weight fast. Problem was our feed schedule was too light. They started to get light and somewhat yellow. We run the three tents and use the same res for all three and kept the EC lower. Looking back we could have had a higher EC in the res and watered down what we were using to water. Hindsight 20/20 sometimes.

That tent is also the hottest of the three since it sits the furthest back in the garage. With the CO2 on it was more manageable. Without it, they've suffered a bit. They seem to perform the best with low-to-mid 80's with lights on and mid-to-high 60's with lights off.

Next run we will mess with it some more, we are leaving a lot on the table, it seems, without the CO2, and that's pretty wild to think about.

 

[brettdawg]

Interesting.
so the tent with co2 wud more than likely yield the most?. Night have to try co2 i used a bag before didnt thonk it wad any good tbf. Wud have to use tank like urs
Yh hard aint it when all feeding out same rez.

 

[CookiesLikeWhoa]

Can't say for certain if it would yield more, since we haven't done it, but considering the buds were bigger earlier on, my guess is yes.

We haven't tried the CO2 bags, but I don't think they get the PPM's up high enough or consistent enough to work. That or you'd have to fill the whole grow space up with those bags. We'd have to run a cost analysis on it. Used to get CO2 for practically free, but times have changed. We move at 1K/lb for the TC, adds another $250/run per tent with the CO2. Break even would be another 1/4lb and profit above that but don't know how much more yield.

Bruce Bungbee has said CO2 can give up to 20% more on a yield. The 'up to' part is what has us worried lol.

 

[Observer]

Interesting.
so the tent with co2 wud more than likely yield the most?. Night have to try co2 i used a bag before didnt thonk it wad any good tbf. Wud have to use tank like urs
Yh hard aint it when all feeding out same rez.

Better off just getting a tank a regulator and a CO2 monitor.

I've not personally used the bags but I think I've seen measurements with them in a tent and not sufficient, or consistent.

 

[Observer]

Interesting.
so the tent with co2 wud more than likely yield the most?. Night have to try co2 i used a bag before didnt thonk it wad any good tbf. Wud have to use tank like urs
Yh hard aint it when all feeding out same rez.

Increased CO2 levels can increase a plants metabolism/photosynthetic rate up to 30% (NetP)

But every other variable needs to be within balance of each other as well to really utilize elevated CO2 levels, efficiently.


pH, E.C, Temperature, Humidity, PPFD, water temperature, leaf surface temperature, optimized spectrums, WC%, DO/root zone oxygenation/O2, wind speed....

Might be missing a few, but that's gotta be most of em.

 

[CookiesLikeWhoa]

The lean is setting in and its getting closer to chop day. 61F for the TC and 66F for the Dante's. It's starting to heat up again in the bay, but only for a few days we hope. Still seeing tons of white pistils in most of the plants.

Trop Cherry: Yeah they're flopping all over the place. Color is coming in, terps are strong and can't wait to get these girls down.



Dante's Inferno: Yeah it's really a fucking mess now. Tons of fat nugs around. Kinda doubted she'd pack on the weight, but the flop tells the story.

Temp: 85F
RH%: 57
PPM: 800
PPFD: 800