The Psyche Gardens

Definitely agree, starting to lean towards root zone temperature and the nozzles not working as they should/ average dropletsize.

The canopy is getting up to 82f, I figure the chamber is probably 75-80 if I leave the door shut, been leaving it cracked now.
How’s the water temperature?
 
What was the last change or two? Although I’m guessing you didn’t wait long enough between changes. Before you saw the jump in roots? How many days in, around 21 for example?
 
What was the last change or two?
keeping around 1-2:30 minutes off, not 10-15 minutes, while they are very young atleast, keeping the nozzles in a good mist/spray pattern.

Proper e.c and not over dosing peroxide or hypo acid too maybe lol.
Although I’m guessing you didn’t wait long enough between changes. Before you saw the jump in roots? How many days in, around 21 for example?
Only takes a few hours to a day or so, to see changes in these kinds of systems, as the roots are just so exposed/ a lot more vulnerable.
 
“The HPA (High-Pressure Aeroponics) method for growing cannabis involves using a nutrient-rich mist to deliver water and nutrients directly to the plant roots. The preferred water droplet temperature in HPA systems typically ranges from 18°C to 22°C (64°F to 72°F). This temperature range helps to optimize nutrient uptake and promotes healthy root development.

Maintaining the water at this temperature can enhance the overall growth and yield of cannabis plants, as it ensures that the roots are not stressed and can efficiently absorb the nutrients provided.”
 
“The HPA (High-Pressure Aeroponics) method for growing cannabis involves using a nutrient-rich mist to deliver water and nutrients directly to the plant roots. The preferred water droplet temperature in HPA systems typically ranges from 18°C to 22°C (64°F to 72°F). This temperature range helps to optimize nutrient uptake and promotes healthy root development.

Maintaining the water at this temperature can enhance the overall growth and yield of cannabis plants, as it ensures that the roots are not stressed and can efficiently absorb the nutrients provided.”
I just might need to get a chiller lol.

Ideally I'd oxygenate RO water to 20-40 mg/l DO.

Have an accumulator tank, solenoid valves, a bigger pump, and micron mesh filters, run around 100 PSI with 10-40 micron antidrip brass nozzles, a inline micron mesh filters as well down to let's say 50 microns, one grower filters down to 1 micron.

Probably would not even need to do inline uv sterilization with DO that high, and recycle my water in a closed loop system.

Make a root trellis/support structure the root system.

Copper mesh lining in the drains or at the bottom or a little above to stop roots growing down into.

although proper misting cycles and they'll stay in the middle/in the mist/aero zone and not grow down anyways.
 
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Fuckin. Cats.

Tote was knocked over for some tens of minutes, 2 died.
the 2 roots were outside of the chamber, doesnt take long at all for them to dry. out.

Here's their replacement.
 

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Looking better now


I lost some trichoblasts though =(
But new ones are forming =)

Some of yesterday's root progression was regressed by cats today or my fault for not having it better secured.

🤷‍♂️

Day 6? Of 12/12 continues.
 

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These don't look too bad though.
 

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Hmm
 

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After fucking with this one nozzle the last 3 fuckin hours, I finally got it spraying correctly.

Lol

It was spraying half way or bad angles out the sides, unevenly, that quick connect fitting there is kinda fucked, and rough getting it in and out (giggity) , yummy microplastics breaking off and getting into that nozzle I think, I take the cap off and let it run, put it back on and it sprays better.
 

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Ahh

Let's see how a cut does In the "system"
 

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interesting


DARPA’s ventures into electric-field and bioelectromagnetic stimulation for plants and microbes have been quite intriguing — though much of their research is still somewhat classified or unpublished in full detail. But here’s what’s generally known or inferred from public info and related research:




DARPA and Electrostimulation: What We Know​


  1. Program Focus:
    DARPA has explored using electric fields, pulsed electromagnetic fields (PEMF), and other bioelectrical methods to enhance plant growth, stress resistance, and microbial activity. The goal: more resilient, faster-growing crops for harsh or controlled environments (e.g., space, battlefields).
  2. Results Highlights:
    • Improved germination and growth rates reported in some trials using low-frequency pulsed electric or magnetic fields.
    • Enhanced nutrient uptake and water efficiency in treated plants.
    • Increased activity in symbiotic microbes in the root zone, which can improve nutrient cycling.
    • Evidence that specific EMF treatments can alter gene expression related to growth and stress response.
  3. Technology Applications:
    • Development of bioelectrochemical systems integrated into hydroponic or aeroponic farms.
    • Exploration of non-chemical growth stimulants reducing fertilizer use.
    • Interest in compact, low-power field generators for autonomous agricultural systems in extreme environments.



Caveats & Reality Check​


  • Results vary widely depending on field strength, frequency, duration, and plant species.
  • The mechanisms are still not fully understood — plant electrophysiology is complex and nonlinear.
  • Scaling from lab to field is challenging; results in controlled environments don’t always replicate outdoors.



Bottom Line:​


DARPA’s research is promising and supports the idea that targeted electric and electromagnetic fields can benefit plant growth and health, but it’s not a magic bullet and requires precise tuning.
 
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academic studies and examples related to DARPA’s interest in electric and magnetic stimulation for plants—these mirror their approach of using electromagnetic fields (EMF) or electric fields to enhance plant growth, nutrient uptake, and stress resistance:




Key Published Studies & Research on Electrostimulation & Magnetics in Plants​


1. Pulsed Electromagnetic Fields (PEMF) Enhancing Plant Growth


  • Study: “Effects of Pulsed Electromagnetic Fields on Seed Germination and Seedling Growth of Tomato (Solanum lycopersicum L.)”
  • Journal: Electromagnetic Biology and Medicine, 2018
  • Summary:
    PEMF exposure (frequency ~50 Hz, field strength 1 mT) increased tomato seed germination rate and improved early seedling vigor by stimulating enzyme activity related to growth.
  • Relevance: Mimics DARPA’s low-frequency PEMF trials aimed at improving plant resilience.



2. Static Magnetic Field Influence on Roots and Nutrient Uptake


  • Study: “Effects of Static Magnetic Field on Growth, Antioxidant Enzyme Activity, and Nutrient Uptake in Soybean Seedlings”
  • Journal: Bioelectromagnetics, 2015
  • Summary:
    Soybean seedlings exposed to 100 mT static magnetic fields for 2 hours daily showed enhanced root length, increased antioxidant activity, and better nutrient uptake efficiency.
  • Relevance: Matches concept of placing plants between N and S magnets for root zone stimulation.



3. Electric Fields Stimulating Root Growth and Oxygen Generation


  • Study: “Electric Field Exposure Enhances Root Growth and Generates Reactive Oxygen Species in Arabidopsis thaliana”
  • Journal: Plant Physiology, 2019
  • Summary:
    Application of low-voltage electric fields (1–5 V/cm) around roots promoted elongation growth and modulated reactive oxygen species (ROS) signaling, which is crucial for root development and defense.
  • Relevance: Demonstrates mechanisms behind your idea of applying low-voltage fields in aeroponic root chambers.



4. Bioelectrochemical Systems and Microbial Interactions


  • Study: “Bioelectrochemical Systems for Microbial Nutrient Recycling in Hydroponics”
  • Journal: Frontiers in Plant Science, 2020
  • Summary:
    Integration of electrodes in hydroponic systems enhanced microbial biofilm activity near roots, improving nitrogen fixation and phosphate solubilization, which boosted plant nutrient availability.
  • Relevance: Similar to DARPA’s vision of bioelectrochemical stimulation of root microbiomes.



5. Magnetic Water Treatment and Plant Growth


  • Study: “Effect of Magnetically Treated Water on Growth and Yield of Tomato Plants”
  • Journal: Agricultural Water Management, 2017
  • Summary:
    Water passed through magnetic fields showed altered physical properties, improving tomato plant growth, leaf area, and yield compared to untreated water.
  • Relevance: Supports claims about magnetic water properties affecting plant hydration and nutrient uptake.



Summary​


Study FocusKey FindingsLink to DARPA Approach
PEMF & Seedling GrowthIncreased germination and early vigorPulsed fields for stress resistance
Static Magnetic Field RootsEnhanced root growth, nutrient uptakeStatic magnets for root stimulation
Electric Fields & RootsRoot elongation and ROS signalingLow-voltage electric stimulation
Bioelectrochemical SystemsImproved microbial nutrient recyclingElectrodes + microbes in hydroponic systems
Magnetic Water TreatmentBetter plant growth via water property changesMagnetically treated irrigation water


 




Testing 5 18 2012 226

""""Sure, the plant on the left was given our "treated" water and the plant on the right got regular RO filtered water. The plant on the left used almost 40% less water overall and about 18% less nutrients. These plants are the same age, same strain, were grown in the EXACT same conditions, only difference being the source water. They were veg'd (in separate systems) for only 18 days. I am looking for the beginning pics of the clones so you can see the growth rates and also that these 2 plants were the exact same size and we even made sure the leaf sets were the same. This test was done over a year ago........"""
 
NPK > MPK + Micronutrients
NPK veg

MKP bloom +micro

CaNO³ -> CaCI² last 7-14 days of flower.

Optional Boosters:

Monosilicic acid (e.g. Power Si) – strengthens cell walls.

PK boosters (some growers add a second PK like 0-50-30 in late flower).

Fulvic/humic acids – aid uptake of micronutrients.



after transition:
ComponentFunction
MKP (0-52-34)Phosphorus & Potassium
Potassium Sulfate (0-0-50)Extra K + Sulfur
Magnesium SulfateMg & S
Trace Micro MixFe, Mn, Zn, B, Mo, Cu
 
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Was reading about dark oxygen and the natural deep sea batteries that make it. Had me wondering if it could be used as a new way to do electro culture. 🤔

But it’s not like the plants sit in the water so…

Getting a few would be impossible anyway for the average person.
 
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On vangst.com


Michigan has 25 job results

Ohio has 79

Colorado has 28


Man fucklahoma, I gotta move.
 

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Was reading about dark oxygen and the natural deep sea batteries that make it. Had me wondering if it could be used as a new way to do electro culture. 🤔

But it’s not like they sit in the water so…

Getting a few would be impossible anyway for the average person.
I was just thinking about those nodules last night...crazy.....and similar thoughts i'm advanced open water certified, but I don't have the equipment to go 2k feet lol


Started watching the why files take on the dark oxygen nodules

I did not know about the voltage they generate then, just the oxygen part but duh, that's from electrolysis.
 
Day 8 or w.e F lol
 

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Tap water that's 7-8 pH, 60-160 ppm
2grams 9-10-28 (AN Sensi Grow Part A salts)
(not a fan of this ratio, especially going into flower, too much nitrogen, and a weird N: P ratio)

1gram mag sulfate
0.7grams pot sulfate.
0.4 grams Citric Acid to pH to 5.8-6.1 for flower.

Unfortunately, nitrogen is pretty bioavaiable the entire pH range we'd be using.



Need to switch to MPK + micros, gotta buy some.

550 ppm/ 1.1 E.c / 5.9 pH 1.5ml hypo acid, 5ml 3% h²o²

SF 2000
3000K warm white
5000K cool white
660nm deep red
760nm infrared (IR)
@ 44 % 450 PPFD~ 8-16 inches above canopy

70RH% / 80 F°
Water temp 75F
2s on / 2:15 off currently.
2 fans on max setting

Need to replace that back quick connect, I think it's just fucking up the nozzle, clogging it with plastic, I think.
 

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