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tobh said:
High level, I plan on doing extracts and tinctures, primarily. Fresh fruit sales just require too much effort but I'd be ok doing custom orders. Secondarily, I plan on getting into spawn production.

Eliminate the need for fruiting conditions and all that crap, just supply larger farms with spawn. The profit margins almost triple by eliminating the secondary stage of spawning to fruit.

Lastly, I will be getting into tissue culturing. I hope to be of value to commercial cannabis producers throughout the state for long term genetic storage and distribution. That's where I think I'll see the highest returns on time investment.
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Good shit man… no doubt you can accomplish that. Your one smart fella.
 
Aqua Man said:
Good shit man… no doubt you can accomplish that. Your one smart fella.
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Half my garage is gonna look like some kind of crazy lab, but it'll all be for above-the-board purposes, most of the time.

Can't have all the cool toys and not experiment with exotic active genetics or do other obscure organic extractions. When in Rome and all that...
 
Battle station is ready. One more component is needed, but I'll fab that up tonight or tomorrow. I have to go get some kind of safety edge, too, as that metal is sharp AF and during the glue up, I didn't set it down perfectly straight so there are some edges waiting to teach lessons
PXL_20230724_032327395.jpg
 
tobh said:
High level, I plan on doing extracts and tinctures, primarily. Fresh fruit sales just require too much effort but I'd be ok doing custom orders. Secondarily, I plan on getting into spawn production.

Eliminate the need for fruiting conditions and all that crap, just supply larger farms with spawn. The profit margins almost triple by eliminating the secondary stage of spawning to fruit.

Lastly, I will be getting into tissue culturing. I hope to be of value to commercial cannabis producers throughout the state for long term genetic storage and distribution. That's where I think I'll see the highest returns on time investment.

End goal is to replace my net salary post expenses and taxes with mycology, tissue culturing, and permaculture CSA gardening.
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Gonna need some hands down the line
 
Gotta get some better incense so I can get a pic of what happens in laminar flow. It's wild, you can watch the smoke organized into a straight stream right from the source. It fights it, but that visible turbulence is caused by the solid material at the origin of the smoke.
 
tobh said:
Alright, table built. I need to secure that sheet metal to the plywood base without screws or nails. Will wood glue work? I have Titebond II Premium. I also have some heavy duty 3M spray adhesive.

Whatchu folks think? Which will provide a better bond?
View attachment 14772

Disregard the polyurethane and brush. Sealing up the back edge that won't have metal on it.
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Not wood glue. It will fail over time. Spray contact cement or other panel bonding glue. You can spread by hand but spray is nice thin layer. Imo
 
Zen_seeker said:
Not wood glue. It will fail over time. Spray contact cement or other panel bonding glue. You can spread by hand but spray is nice thin layer. Imo
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Ended up using spray, and fucked it up in the process. The setup on the spray is crazy fast, so the initial lay of the metal was off and I had to peel it back off then re-lay.

All good, though. Now I can measure and get a piece of glass cut to put on the metal, and finish the table off for years to come.
 
Lesson learned: if you're gonna lay a fraction of an inch worth of metal on heavy duty, fast acting glue, do it with a second set of hands. Or do it in sections so the metal lays true.
 
First experiment. Goal is to determine effectiveness of the filter.

Three samples taken. Left to right is open air in the same room the filter runs in, 1hr sample in laminar flow, and 2hr sample in laminar flow. Left and center were taken at the same time.

Each sample was taken by laying the petri dish open horizontally, undisturbed for a duration of 10 minutes. Each dish was wiped down thoroughly with 70% ISO prior to opening in laminar flow.

These will go back in my incubator and checked daily for any signs of growth.

Worth noting: these plates are over two months old and have been in my incubator. I have reasonable trust they are clean and will not show contaminate outside that which is present through this experiment.
PXL_20230724_055214526.jpg
 
tobh said:
First experiment. Goal is to determine effectiveness of the filter.

Three samples taken. Left to right is open air in the same room the filter runs in, 1hr sample in laminar flow, and 2hr sample in laminar flow. Left and center were taken at the same time.

Each sample was taken by laying the petri dish open horizontally, undisturbed for a duration of 10 minutes. Each dish was wiped down thoroughly with 70% ISO prior to opening in laminar flow.

These will go back in my incubator and checked daily for any signs of growth.

Worth noting: these plates are over two months old and have been in my incubator. I have reasonable trust they are clean and will not show contaminate outside that which is present through this experiment.
View attachment 14794
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Awesome
 
Here we go. First batch of agar getting ready to be cooked up in the new house, in the front of the new hood.

Keeping it simple this time. 9.5g agar, 9.5g light malt extract in 500ml water.

PXL_20230726_025622292.jpg
 
Plates are poured. The glasses have hot water in them to help eliminate condensation forming on the lids.

Gotta say, pouring this way is a hell of a lot easier than in a SAB. Gotta work on the technique as I should've been able to get 25 plates out of that 500ml, but I only got 21, so gotta get more accurate with the pours .

They'll live in front of the hood until cool, then tomorrow I'll be doing some transfers and some new inoculations to get cultures going.

PXL_20230726_050550116.jpg
 
Need to get some soft, sticky rubber feet for the cooling racks. The frequency of the vibration from the blower moves them, and whatever is on them.

Real time learnings, hope it helps someone later on.

Edit: removed glasses to rotate the plates, and this little condensation collection greeted me
PXL_20230726_053829524.jpg
 
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tobh said:
Need to get some soft, sticky rubber feet for the cooling racks. The frequency of the vibration from the blower moves them, and whatever is on them.

Real time learnings, hope it helps someone later on.

Edit: removed glasses to rotate the plates, and this little condensation collection greeted me
View attachment 15109
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love this shit.
 
I Think the extra heat from this dogshit building apartment/management/undersized HVAC systems/non-existing insulation or pisspoor

the heat is just fucking it up, allowing mold and other contams to grow, +possibly too high water content

and besides my sterility, and fucking with the stuff, i have been better at leaving the alone, i just check on them and leave em be.

that jar with the healthy looking mycellium showed mold today. along with the Uncle bens bag.


i will probably pick this back up when i move.

i dont see how i can do this when temps are so high and unstable/non-consistent.
 
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Observer said:
I Think the extra heat from this dogshit building apartment/management/undersized HVAC systems/non-existing insulation or pisspoor

the heat is just fucking it up, allowing mold and other contams to grow, +possibly too high water content

and besides my sterility, and fucking with the stuff, i have been better at leaving the alone, i just check on them and leave em be.

that jar with the healthy looking mycellium showed mold today. along with the Uncle bens bags.


i will probably pick this back up when i move.

i dont see how i can do this when temps are so high and unstable/non-consistent.
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You can do it, but there's a price tag associated with it.

Basic equipment:
  • pressure cooker/canner: $80
  • mason jars: $15

IIRC you already have a SAB, so you have your workspace. This is one of those hobbies where trying to go super budget ends up being way more expensive. With the right, core pieces of equipment, you can do this anywhere, in any conditions.

There's no need to go big or extravagant, unless you know this won't be a passing thing. You got this dude, just go back to the basics. Do some PF Tek runs, get your feet wet, figure out the process, then build from there.

When I started, my SAB was a cardboard box with a Saran wrap top. I blew my eyebrows off by lighting a lighter in it to sterilize a needle with a shit ton of Lysol vapor in it. I used my mom's little 4 quart PC for years to sterilize jars. I got most everything for free from local farms -- grains, horse shit, old containers... And I supported myself through high school and college doing it that way.

All that being said, not having a cheap, small pressure cooker is your Achilles heel right now. Get that and you can do this.
 
Observer said:
why do you not want condensate to form?
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Excess water can harbor bacterial contamination and makes it hella hard to observe what's going on inside the plate.

When working with agar you want super clear pours -- it should look like piss colored glass -- and super clear lids. That way you can clearly and easily see what's going on, on the surface.

Many bacterias aren't colorful or thick. They'll present as what looks like water spots on paint, or snail trails on concrete. Just a slight, roughed up squiggly line. With condensate present, it makes seeing those infections very, very difficult.
 
Just staring at my containers wondering how well I’m going to manage contamination. Seems like it’s most of the battle and a constant fight.

Then environment. Heat seems to be the catalyst in Observers case. Due to unforeseen circumstances.

What is the perfect temperature and RH to keep the containers in? Is it better in a dark dry but cool place or slightly warm spot? Should it have air flow or none at all?

I really expected Observer to get results by now. Is this just bad luck at trying to make cheap solutions work or is this just the price you pay when starting cheap without the basics like Tobh hinted at?

Is this a better container setup @Aqua Man or just stick with what you showed us? I haven’t drilled any holes yet. I’m not worried about cracking it, just getting proper air flow without getting complicated.
 
@Observer hang in there man. You got a lot of shit going down this month. Maybe a break until you get to the new pad is the right step. Just don’t get discouraged. These guys have been doing this a lot longer with much better gear these days.

You keeping notes on where things start to go wrong and why? I even journal my grow so plan to add mushrooms to it or start a new one. Need to get repeatable steps to known outcomes and expected results.

Looking forward to seeing your first successful grow man. 🍿👍
 
Zen_seeker said:
Just staring at my containers wondering how well I’m going to manage contamination. Seems like it’s most of the battle and a constant fight.

Then environment. Heat seems to be the catalyst in Observers case. Due to unforeseen circumstances.

What is the perfect temperature and RH to keep the containers in? Is it better in a dark dry but cool place or slightly warm spot? Should it have air flow or none at all?

I really expected Observer to get results by now. Is this just bad luck at trying to make cheap solutions work or is this just the price you pay when starting cheap without the basics like Tobh hinted at?

Is this a better container setup @Aqua Man or just stick with what you showed us? I haven’t drilled any holes yet. I’m not worried about cracking it, just getting proper air flow without getting complicated.
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75-80f colonization and 70-75f fruiting. In a mono tub humidity isn’t to big of a concerns but 85-95% in the tub is a good place for colonization 80-90% fruiting. You need to have evaporation from the surface in fruiting but still maintain moisture. Thats the balancing act with FAE holes
 
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