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I use just straight coco coir. I’ve tried messing around with recipes adding gypsum and vermiculite but I really see no difference.

I just put spores to plate last night, Got some Melmac Reverts and PE#6 started.
Im doing coco verm. The verm lowers the cost a bit. Small tubs atm but probably get back into the bigger monos soon.

200g coco
1 quart verm
5 cups water

Perfect field capacity everytime.
 
Im doing coco verm. The verm lowers the cost a bit. Small tubs atm but probably get back into the bigger monos soon.

200g coco
1 quart verm
5 cups water

Perfect field capacity everytime.
I usually run 6, 12qts at a time. I learn with straight coco a 650g brick and 3.5 quarts of water hits field capacity perfect for the whole brick.
 
Some Amazon just starting to fruit.
 

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I usually run 6, 12qts at a time. I learn with straight coco a 650g brick and 3.5 quarts of water hits field capacity perfect for the whole brick.
Yeah i have dropped down to about 3 quarts spawn to the mix i listed about probably 3-4 quarts as i never measured. So you are running pretty much what i used to.

The PE i have is so contam prone i dropped down. But this Amazon and B+ Im just starting seem super easy and very contam resistant so i will probably jump back up after a couple tubs
 
Yeah i have dropped down to about 3 quarts spawn to the mix i listed about probably 3-4 quarts as i never measured. So you are running pretty much what i used to.

The PE i have is so contam prone i dropped down. But this Amazon and B+ Im just starting seem super easy and very contam resistant so i will probably jump back up after a couple tubs
i remember back in circa 2001 when I started, B+ was the most recommended strain for beginners to start with. Granted, the PF Tek was also the recommended method and people that did bulk casings were super close lipped about their methods and techniques. However, anything that's technically a mutation is gonna be more susceptible to contams just by the nature of being a mutation.

still have some APE that I need to get into some grains, it's just such a slow colonizer I haven't bothered to fuck with it beyond getting a clean culture on agar.
 
i remember back in circa 2001 when I started, B+ was the most recommended strain for beginners to start with. Granted, the PF Tek was also the recommended method and people that did bulk casings were super close lipped about their methods and techniques. However, anything that's technically a mutation is gonna be more susceptible to contams just by the nature of being a mutation.

still have some APE that I need to get into some grains, it's just such a slow colonizer I haven't bothered to fuck with it beyond getting a clean culture on agar.
Yeah that PE takes 3 times as long to colonize and 3x as long to fruit…. At min. But its very fucking potent. And I can’t keep it around more than a day or 2.

I will save that for certain people but its such a pita its almost not worth it.

The next atep i am going to do for just knowledge will be a stain called holy grail its an enigma that takes about 6 months.

But i need to build up some patience before i go there lol
 
Yeah that PE takes 3 times as long to colonize and 3x as long to fruit…. At min. But its very fucking potent. And I can’t keep it around more than a day or 2.

I will save that for certain people but its such a pita its almost not worth it.

The next atep i am going to do for just knowledge will be a stain called holy grail its an enigma that takes about 6 months.

But i need to build up some patience before i go there lol
jeez lol that's almost as bad as psilocybe cyanescens. which, reminds me. i need to move forward with getting the spawn expanded on those and get em out to some outdoor beds. maybe by next year will end up with a crop of wood lovers.
 
Figured I'd show how i go about making agar plates. This recipe is about as simple as it can get and works effectively for most fungal cultures.

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To start, measure 500ml warm water (cold will make the agar agar powder and the light malt extract clump together and not mix as effectively) in your chosen flask. I use a borosilica high-temp rated Erlenmeyer flask; some use whisky bottles, or even jars. Place a magnetic stir stick in the water.
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Now, into the flask weigh out 10g LME (light malt extract) and 9g agar agar powder. You can do a 1:1 ratio of LME:agar agar or other variations. I find 10g of agar agar with this particular brand to harden up a bit too much once poured so I vary between 8g and 9g most of the time now. Add the two powders and set on your magnetic stirrer for a few minutes. I don't really measure this time as each batch is different, you just want to ensure the LME and agar agar powder are thoroughly mixed in the solution.
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^ agar agar powder

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^ Light Malt Extract. It's worth noting that LME is hygroscopic. If you even THINK about moisture at all, it will clump and get super sticky -- like kinetic sand that becomes glue.

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Place your cap or cover on your container and tighten to closed position, then back off a 1/4 turn. This ensures that pressure build up doesn't turn your container into a bomb inside the PC. I cover mine with foil as a force of habit; not sure it's really necessary though.

Place your container in the PC and fill with water to the same height as the agar solution in your container. This is to help reduce the chances of boiling over as the solution cools off after sterilization.

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^ Flask in water bath. The empty, no-lid jars are there to prevent the flask from tipping over while cooking.

Place the PC on your burner (if using one that requires external heat sources) and crank the heat as high as you can. You want to bring temps up as quickly as possible at this point so reduce the chances of caramelizing the sugars in the LME. Once you see steam coming out of the weight vent, set a timer for ten minutes. The idea is to purge all the air in the PC with steam.

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When the 10m timer goes off, set your weight on the vent, and keep the heat up to get to 15psi as quickly as possible. Once the weight wobbles, reduce the heat and set a timer for 20 minutes. You want the heat to be high enough to maintain pressure but only so high to cause the weight to jiggle once or twice per minute. Any more than that and you're running too high of temperatures, and caramelizing sugars, any lower and you're not maintaining 15psi for adequate sterilization.

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When the 20m timer goes off, shut off the heat. If you have a flow hood now is a good time to move the PC into that clean stream of air. As the PC cools, it creates a vacuum and has the potential of pulling in contaminates -- contaminates right into the sterile environment you just created.

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Allow the PC to cool until the pressure lock drops, and carefully (using a silicon oven mitt) remove the container of agar solution from the cooker and set in your clean stream of air. At this point you should start monitoring the temperature of the agar solution with an infrared thermometer. Agar begins to solidify at approximately 122F (50C), and ideal pouring temperature is 131F (55C).

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You will have to move quickly when the agar reaches that target temperature because the temperature really starts to drop at that point. I like to separate my stack of plates into two stacks of ten (500ml makes ~20 plates), and starting at the bottom of one stack, lift the lid and the entire stack, pour just enough to cover the entire bottom of the plate, replace the lid and move up the stack. With practice you'll get pretty good at this.

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^ plates poured. The jars have hot water in them which helps prevent condensation from forming on the lids of the plates.

Allow the plates to cool for a few hours before wrapping with parafilm or inoculating with your chosen culture (spores, mycelium, fruit body, etc). Made plates can be stored in open air if wrapped with parafilm for up to a month, and even longer (I've kept some as long as six months) in refrigeration.

It's worth noting I did not cover additional sterile procedures. I assume if you're doing this, you know how to practice aseptic techniques and have all the appropriate equipment and supplies to successfully perform this process.

Happy mushrooming!
 
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how similar are the pan vs psi with end use? I lived in the pnw for a while and the psilocybe cyanescens were everywhere.
 
how similar are the pan vs psi with end use? I lived in the pnw for a while and the psilocybe cyanescens were everywhere.
psilocybe cyanescens is a wood lover, and only slightly more potent than cubensis. However -- it has this wonderful side effect called Wood Lover's Paralysis. Medical and mycological science doesn't quite understand why they cause temporary paralysis but it is a known side effect. Some independent research has shown benadryl can help alleviate or prevent the symptoms from presenting themselves but there hasn't been any academic or otherwise research done (or at least published) to determine exactly what causes the paralysis.

pan cyans are dung lovers, and are substantially more potent than psilocybe cubensis. The quantities of psilocin and psilocybin are exceedingly high per volume in the fruit bodies. However, they're also much smaller fruits. Typical dosing recommendations are ~1g pan cyan is equivalent to 4-5g cubensis. They're also reported to cause less body load and offer a more enjoyable experience than cubensis as the common gut issues inherent to cubensis aren't present. Some schools of thougth say this is due to the user consuming less material, and thus being exposed to less of the alkaloids and other compounds that negatively impact the gut by way of consuming raw mushrooms. My thinking is there is likely some type of hystamine that causes much of the stomach upset typically experienced with cubensis and other edible mushrooms when eaten raw.
 
Yeah that PE takes 3 times as long to colonize and 3x as long to fruit…. At min. But its very fucking potent. And I can’t keep it around more than a day or 2.

I will save that for certain people but its such a pita its almost not worth it.

The next atep i am going to do for just knowledge will be a stain called holy grail its an enigma that takes about 6 months.

But i need to build up some patience before i go there lol
Man I'd love to try those potent ones....
 
i remember back in circa 2001 when I started, B+ was the most recommended strain for beginners to start with. Granted, the PF Tek was also the recommended method and people that did bulk casings were super close lipped about their methods and techniques. However, anything that's technically a mutation is gonna be more susceptible to contams just by the nature of being a mutation.

still have some APE that I need to get into some grains, it's just such a slow colonizer I haven't bothered to fuck with it beyond getting a clean culture on agar.
APEs they are a fun novelty grow is how I see them, I have noticed one things about them they do tend to colonize faster on potato dextrose agar plates instead of LME. Last run I did the Yeti mutation and damn they are finicky little things.
 

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APEs they are a fun novelty grow is how I see them, I have noticed one things about them they do tend to colonize faster on potato dextrose agar plates instead of LME. Last run I did the Yeti mutation and damn they are finicky little things.
ah good call out. i haven't ran em on PDA yet, just LMEA and nutrient yeast LMEA. They seemed move a little faster with the nutrient yeast but not enough to be excited about.
 
New prints arrived today. Giving the ol pan cyans one last go before marking that goal as a not gonna happen on the list of experiments to perform.

Varieties are:
Pan Cyan Australia Red Down Under
Pan Cyan Central Florida
Pan Cyan Australia
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Heck yes, 150lbs of rye berries showed up today. Came out to about $35 per 50lb bag. Way more economical than sourcing smaller amounts from myco suppliers or health food stores.

Quality is yet to be determined, but I'm hopeful this new supplier will help keep the investment costs lower as my operation scales.
 
Heck yes, 150lbs of rye berries showed up today. Came out to about $35 per 50lb bag. Way more economical than sourcing smaller amounts from myco suppliers or health food stores.

Quality is yet to be determined, but I'm hopeful this new supplier will help keep the investment costs lower as my operation scales.
This is the one medium I’ve yet to try for colonizing spawn. Does it colonize faster?

About to spawn this Rusty Whyte, going to take a few grains and put them to agar just incase it’s something special.

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This is the one medium I’ve yet to try for colonizing spawn. Does it colonize faster?
I haven't noticed any increase in speed vs WBS. It's been too long since I last did any BRF cakes, either, so can't give a reliable opinion on that either. But, I like the preparation better, and the grain size better than WBS or popcorn. It's a little more tedious than popcorn but not nearly the PITA that WBS is. I have some ideas to reduce the issues I have with prep (primarily drying the grain before loading it into jars). The nutrient density is also fantastic for pretty much all the varieties of mushrooms I'll be producing in bulk (cordyceps will be a whole other animal to tackle later on), and that's helpful for keeping the complications down.

I may pick up some whole oats to try as a side by side as I'll be working through this stock of rye for a while, but will likely stick with rye for the foreseeable future. Once I work out the kinks for producing very large (relative to my scale) batches of grain at once, I'll be able to train up the kids and the ol lady on that part of the process and just let them run that so I can focus on the lab work and optimizing the next segments of the production pipeline.
 
Meant to attach this to the original post, ran past the time limit though
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Heck yes, 150lbs of rye berries showed up today. Came out to about $35 per 50lb bag. Way more economical than sourcing smaller amounts from myco suppliers or health food stores.

Quality is yet to be determined, but I'm hopeful this new supplier will help keep the investment costs lower as my operation scales.
I just got a source for $25 per 50/lb bag. Things are ramping up now 😁 lost my contact that was $40 when i moved. My first 2 bags after coming back cost me almost $200 to get shipped in. Picked up 4 bags for now and see where that takes me. Supplier always has but i can get it even cheaper if i can buy in totes. Lmao thats a lot of mushrooms to grow to need that much
 
I just got a source for $25 per 50/lb bag. Things are ramping up now 😁 lost my contact that was $40 when i moved. My first 2 bags after coming back cost me almost $200 to get shipped in. Picked up 4 bags for now and see where that takes me. Supplier always has but i can get it even cheaper if i can buy in totes. Lmao thats a lot of mushrooms to grow to need that much
hell yeah it is lol i already hit up a buddy in my old town that grows for a seasonal farmers market out there and asked if he wanted to take one of the bags off my hands. it's a lot of grain to deal with, man. even more to prep and process through a medium sized PC. Gonna have to build a steam sterilizer barrel system me thinks.
 
hell yeah it is lol i already hit up a buddy in my old town that grows for a seasonal farmers market out there and asked if he wanted to take one of the bags off my hands. it's a lot of grain to deal with, man. even more to prep and process through a medium sized PC. Gonna have to build a steam sterilizer barrel system me thinks.
Yeah i can do 7 jars at a time. Right now that eats up a lot of my Saturday. I was thinking of getting another for now.

These Amazon are very easy growers and very vigorous. Haven’t tried them yet but i just finished drying the first of them so maybe test this weekend.

They won’t be PE but they are so dam fast and easy
 
Yeah i can do 7 jars at a time. Right now that eats up a lot of my Saturday. I was thinking of getting another for now.

These Amazon are very easy growers and very vigorous. Haven’t tried them yet but i just finished drying the first of them so maybe test this weekend.

They won’t be PE but they are so dam fast and easy
that's what i remember from growing them years ago. they're not quite as aggressive as KSSS but they're up there. I have a jar of JMF and Pink Buffalo going right now that I'll be propagating out to more grain bags probably tomorrow, and need to make some test plates for their respective LCs I made. also still gotta go find some containers to actually fruit the things in.

was gonna use seedling trays but after I measured the ones I have they're only about 2.75" deep and I would much rather have 4"-6" of substrate just to maximize the size of the fruit bodies. This will also be the first run through the martha tent, so want to make sure I have everything dialed in I can and only have to focus on fine tuning the environment.
 
that's what i remember from growing them years ago. they're not quite as aggressive as KSSS but they're up there. I have a jar of JMF and Pink Buffalo going right now that I'll be propagating out to more grain bags probably tomorrow, and need to make some test plates for their respective LCs I made. also still gotta go find some containers to actually fruit the things in.

was gonna use seedling trays but after I measured the ones I have they're only about 2.75" deep and I would much rather have 4"-6" of substrate just to maximize the size of the fruit bodies. This will also be the first run through the martha tent, so want to make sure I have everything dialed in I can and only have to focus on fine tuning the environment.
I tried seedling trays and they suck ass bro.

I like 3.5” depth for the same reason.

@Dirtbag and I both grew KSSS also that shit was the fastest i have seen. This is close but not quite as you say. I liked the KSSS also but i think the amazon are a bit stronger? Well i hope
 
The grain was just shaken yesterday, but the recovery is great. Especially considering those grain jars have been stored for months. Was just an experiment to see what the shelf life of sterilized grain might be, and it seems to be performing nearly as good as if it were prepped yesterday.
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Yeah i can do 7 jars at a time. Right now that eats up a lot of my Saturday. I was thinking of getting another for now.

These Amazon are very easy growers and very vigorous. Haven’t tried them yet but i just finished drying the first of them so maybe test this weekend.

They won’t be PE but they are so dam fast and easy
If you want a high potency mushroom without the long agonizing growth rate of PE try some True Albino Teachers. I can’t tell much difference in growth than a regular cube, but with buckle the fuck up potency. Every time I harvest them they turn black instead of blue.
 
I tried seedling trays and they suck ass bro.

I like 3.5” depth for the same reason.

@Dirtbag and I both grew KSSS also that shit was the fastest i have seen. This is close but not quite as you say. I liked the KSSS also but i think the amazon are a bit stronger? Well i hope
KSSS is one of my all time favorites. Haven't been able to find a reliable source for a print again, yet, but I'll find it eventually. I liked them for their aggressive growth rates, size of fruits, and strong potency. In comparison to like, Thai Lipa Yai, they definitely carried more of a holistic punch. Thai Lipa Yai was super visual without the same kind of body load. We'll see what the Jedi Mind Fuck are like. It's my first venture into the new school hybrid varieties. Everything else I've done before has been some regional variety.
 
If you want a high potency mushroom without the long agonizing growth rate of PE try some True Albino Teachers. I can’t tell much difference in growth than a regular cube, but with buckle the fuck up potency. Every time I harvest them they turn black instead of blue.
Ok im going to order them tomorrow. I’m ditching the PE entirely. I have done 3 grain transfers of amazon and the PE is just finishing colonizing the first round.

The B+ are fast too but a few days behind the amazon. As far as what i hear the amazon is stronger.

I have grown regular GT’s and they were fast and reliable but the potency was mediocre.

Now let me go see if they have them.
 
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