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All things shrooms

looks like i'll be spawning the JMF tomorrow. the spawn is starting to consolidate and only about 5% of outer grains in one bag isn't fully colonized yet.

a little concerned with the pink buffalo, one bag looks like it's starting to go bacterial but can't smell that signature smell through the filter patch, yet, so letting it run until proven ok or not. if i did lose one bag to bacteria, definitely gonna be evaluating where the contam is coming from, though it very well may be I just slipped up with my aseptic technique.

the pink oyster is roaring, too. already at 20% colonization from what I can tell. will probably be ready to expand out to the next set of bags by this weekend. will plan on getting the remaining plates into bags at the same time, make for a solid sunday fun day in the lab.

in the interim i've gotta get back out there and mix up the straw+cvg, get it into bags, and get pasteurizing. the plan of matching weight to weight for the straw ain't gonna work, so going by volume instead. for this first round, gonna do 1:1 cvg:straw, and will modify from that baseline depending on how these first tubs run. should about double the volume of substrate i'm sitting on right now either way.
 
SpectrumFarms said:
Update on the Drippy Popcorn Tek. Seems to work out pretty well so far, this was inoculated 10 days ago with 1/4 of an agar dish. This a test batch of Melmac Revert, I did notice over the days it does seem to start tiny inoculation points all over the medium much faster. I started to notice on day 3. Couple more days to full send.
View attachment 67874View attachment 67875
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Gonna try this when I get a PC
 
This is kind of comical. Pretty sure this pot is near the limit of what the glass top is rated for on my range. But, pasteurizing is gonna get done either way. Ran out of daylight waiting on the straw to hydrate and didn't want to babysit the propane burner.
PXL_20240806_041537507.jpg
 
tobh said:
looks like i'll be spawning the JMF tomorrow. the spawn is starting to consolidate and only about 5% of outer grains in one bag isn't fully colonized yet.

a little concerned with the pink buffalo, one bag looks like it's starting to go bacterial but can't smell that signature smell through the filter patch, yet, so letting it run until proven ok or not. if i did lose one bag to bacteria, definitely gonna be evaluating where the contam is coming from, though it very well may be I just slipped up with my aseptic technique.

the pink oyster is roaring, too. already at 20% colonization from what I can tell. will probably be ready to expand out to the next set of bags by this weekend. will plan on getting the remaining plates into bags at the same time, make for a solid sunday fun day in the lab.

in the interim i've gotta get back out there and mix up the straw+cvg, get it into bags, and get pasteurizing. the plan of matching weight to weight for the straw ain't gonna work, so going by volume instead. for this first round, gonna do 1:1 cvg:straw, and will modify from that baseline depending on how these first tubs run. should about double the volume of substrate i'm sitting on right now either way.
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When in doubt throw it out bro… i have sone it to many times where i’m like hmmm not sure i will try it.

Think i am about 100% contam everytime i did that lol
 
Aqua Man said:
When in doubt throw it out bro… i have sone it to many times where i’m like hmmm not sure i will try it.

Think i am about 100% contam everytime i did that lol
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that's probably what I'm gonna end up doing. it's a setback but can't win em all. honestly, i would be pretty surprised if something hadn't contaminated at this point. too many hours have been spent in front of that hood for everything to have been done right lately haha
 
Inaugural trays are in the Martha. The journey to this point has taken a couple years but, we have arrived!

Going forward, I absolutely want horse manure. The volume of substrate isn't quite where I wanted it; about a half inch lower in the trays than planned. I'm hoping with having constant FAE going that fuzzy feet doesn't set in, otherwise I've considered setting up some 120mm waterproof computer fans on each shelf at substrate level to move the CO2 off the surface of the trays.

We'll see, though! Will check the trays in a week, and determine when the covers will come off and the humidifier will go on.
PXL_20240807_004900147.jpgPXL_20240807_005614339.jpg
 
tobh said:
Inaugural trays are in the Martha. The journey to this point has taken a couple years but, we have arrived!

Going forward, I absolutely want horse manure. The volume of substrate isn't quite where I wanted it; about a half inch lower in the trays than planned. I'm hoping with having constant FAE going that fuzzy feet doesn't set in, otherwise I've considered setting up some 120mm waterproof computer fans on each shelf at substrate level to move the CO2 off the surface of the trays.

We'll see, though! Will check the trays in a week, and determine when the covers will come off and the humidifier will go on.
View attachment 68062View attachment 68063
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Looks great man
 
tobh said:
🤞for some good cloning candidates. would be cool to get to a point of even, dense canopies. need to do more research about getting to that level of monoculture though.
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Just have to keep going till you find those fruiting traits… luck of the spore 🤣
 
So, i almost threw away the APE bag I inoculated MSS. Was just checking on the agar plates, and the current grain bags, and it finally decided to show signs of germination. Was inoculated on 7/25, so 13 days to germinate. Of the agar plates, only one has shown germination but is moving predictably slow.

Definitely need to get the lion's mane plates onto some grain, and pretty much all of the pan cyans are ready to go on grain too.

The pink buffalo seems to be moving again, but I'm watching real closely given I suspect bacterial infections are present. Still smells like grain through the filter patch, not that signature sweet stink of bacteria, but I'll have to grab some pics of the suspect indicators of bacteria. I'll probably knock up a new master bag of grain with the LC either way because the time sink of uncertainty isn't worth it when a sure thing is available.

Should've grabbed some pics of the JMF bags and the pink oyster side by side before spawning yesterday. The density of the mycelium is way different. Oyster mycelium almost looks like cobweb whereas psilocybe mycelium is way more dense. I know it's not cobweb as I cloned from a fruit on a petri dish, but it's such a difference that its worth documenting digitally.

Looking at getting some test tubes to make slants. I'll need a rack to go in the PC too. Any suggestions anyone? Looking to do some long term storage of genetics, and petri dishes aren't quite ideal for that.
 
tobh said:
Looking at getting some test tubes to make slants. I'll need a rack to go in the PC too. Any suggestions anyone? Looking to do some long term storage of genetics, and petri dishes aren't quite ideal for that.
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I used to use the short vials that they use to take blood samples. About 3”-4” long with a red/brown rubber top. You can push a syringe through if needed and it self seals.
 
Zen_seeker said:
I used to use the short vials that they use to take blood samples. About 3”-4” long with a red/brown rubber top. You can push a syringe through if needed and it self seals.
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i was looking at those, a bit too rich for my blood right now lol at least the ones i was finding. vacutainers -- they be the bougie clandestine mycologist's choice haha. wasn't looking to spend $100+ for more test tubes than I'll likely use before i break em in the next five years. ended up with some centrifugal poly ones that will hold up in the PC.
 
tobh said:
i was looking at those, a bit too rich for my blood right now lol at least the ones i was finding. vacutainers -- they be the bougie clandestine mycologist's choice haha. wasn't looking to spend $100+ for more test tubes than I'll likely use before i break em in the next five years. ended up with some centrifugal poly ones that will hold up in the PC.
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Helps when you have family that work at hospital. They toss out a lot of new items because the box was open even if not used. The waste is nuts. I get the reasons behind not using open boxes and bottles but maybe have smaller portions/containers. 🤷‍♂️
 
Zen_seeker said:
Helps when you have family that work at hospital. They toss out a lot of new items because the box was open even if not used. The waste is nuts. I get the reasons behind not using open boxes and bottles but maybe have smaller portions/containers. 🤷‍♂️
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but then how are they gonna charge the common man $10k for a bag of saline?! we gotta think about the board of directors man. they can't go suffering due to our savings! 😂
 
Aqua Man said:
Moved on to some big tubs. May post pics when they start to fruit.

@Zen_seeker was it you i was going to get some spawn jars to a long time ago?

I finally up and running at a decent level now that i dropped the PE. If so i have jars ready to send
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are you doing monotubs now or what's the deal?

i'm having a real hard time not peaking at the tubs lol it's been three days but it feels like an eternity.
 
tobh said:
are you doing monotubs now or what's the deal?
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Yeah mid size small enough they fit my racks. Not as big as way back but decent enough. Dropped my spawn ratio on then a fair bit so will see but so far the first is looking pretty well for coming home and doing it after drinking for 12 hrs.

Lol i usually try to avoid those kinda shenanigans but you know how it goes.

I can’t count how many mishaps i have had thinking its a great idea to fuck with my stuff at the time.
 
Aqua Man said:
Yeah mid size small enough they fit my racks. Not as big as way back but decent enough. Dropped my spawn ratio on then a fair bit so will see but so far the first is looking pretty well for coming home and doing it after drinking for 12 hrs.

Lol i usually try to avoid those kinda shenanigans but you know how it goes.

I can’t count how many mishaps i have had thinking its a great idea to fuck with my stuff at the time.
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haha you're telling me. i was talking to an old friend of mine that remembers way back when i started growing about how given the equipment and methods i use now, it's amazing we ever were able to enjoy any fruits of those labors back then. so much unsanitary work in non-ideal environments, all the time. granted, i was in the high desert back then and mold counts were almost non-existent, my worst contams were bacterial, but trich would hit spent monotubs after the fifth flush almost every time.

now i just try not to fuck with anything if it doesn't need fucked with. kinda like cannabis. too much love is not a good thing. but, i'm genuinely curious because the martha is new, i've not done tubs like this before, new substrate mix, and i'm not 100% sure i had the substrate saturated quite enough so i'm just hoping when i pop those covers in four days that things look good and i didn't waste 10lbs of spawn lol
 
tobh said:
haha you're telling me. i was talking to an old friend of mine that remembers way back when i started growing about how given the equipment and methods i use now, it's amazing we ever were able to enjoy any fruits of those labors back then. so much unsanitary work in non-ideal environments, all the time. granted, i was in the high desert back then and mold counts were almost non-existent, my worst contams were bacterial, but trich would hit spent monotubs after the fifth flush almost every time.

now i just try not to fuck with anything if it doesn't need fucked with. kinda like cannabis. too much love is not a good thing. but, i'm genuinely curious because the martha is new, i've not done tubs like this before, new substrate mix, and i'm not 100% sure i had the substrate saturated quite enough so i'm just hoping when i pop those covers in four days that things look good and i didn't waste 10lbs of spawn lol
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Yeah that gonna take some tinkering especially with that manure… pasteurized and not sterilized is IMO a key element unless you have a fast colonizer or very sterile environment.

I have carpets in my fruiting room… it just doesn’t cut it for slow growers. But these B+ and amazon are fucking bulletproof.

Once i see more if a return i will build a sterile room i have most of the material but it also requires more equipment so yeah
 
Aqua Man said:
Yeah that gonna take some tinkering especially with that manure… pasteurized and not sterilized is IMO a key element unless you have a fast colonizer or very sterile environment.

I have carpets in my fruiting room… it just doesn’t cut it for slow growers. But these B+ and amazon are fucking bulletproof.

Once i see more if a return i will build a sterile room i have most of the material but it also requires more equipment so yeah
Click to expand...
yeah, i don't have the manure yet, so this round is just straw + cvg but straw is so damn contam happy and i have a lot of mold around in my piece of the forest. that was a major reason i actually did the mixing of substrate and spawn in front of the flow hood. Never in my life have I done that step with such sterile conditions.

I also tried a new method of pasteurizing, which I'll not be doing again because it was just too much of a PITA. going back to pillow cases unless i can find some other porous material containment that will hold decent volumes of material until i can get a steam sterilizer built. I used myco bags in a water bath @ 145F for two hours. the buoyancy of the material was a challenge to keep weighed down and the pot had more dead space that i would've liked.

i designed a larger than common unit of the Bubba Barrel. Basically it's a large horse trough on its side, and a boiler barrel to produce the steam. figuring out the safety valve sizing and whatnot is still in the works, but i figure something of that size should be able to process 250lbs+ of material every 24 hours. if i manage to get to the scale i want to get to, that'll probably be enough to make it through a week at a time.

all in all, figuring shit out for future scale, and having a bit of fun in the meantime. gotta get grains soaking after work today so there are enough bags ready to go over the next week.
 
tobh said:
yeah, i don't have the manure yet, so this round is just straw + cvg but straw is so damn contam happy and i have a lot of mold around in my piece of the forest. that was a major reason i actually did the mixing of substrate and spawn in front of the flow hood. Never in my life have I done that step with such sterile conditions.

I also tried a new method of pasteurizing, which I'll not be doing again because it was just too much of a PITA. going back to pillow cases unless i can find some other porous material containment that will hold decent volumes of material until i can get a steam sterilizer built. I used myco bags in a water bath @ 145F for two hours. the buoyancy of the material was a challenge to keep weighed down and the pot had more dead space that i would've liked.

i designed a larger than common unit of the Bubba Barrel. Basically it's a large horse trough on its side, and a boiler barrel to produce the steam. figuring out the safety valve sizing and whatnot is still in the works, but i figure something of that size should be able to process 250lbs+ of material every 24 hours. if i manage to get to the scale i want to get to, that'll probably be enough to make it through a week at a time.

all in all, figuring shit out for future scale, and having a bit of fun in the meantime. gotta get grains soaking after work today so there are enough bags ready to go over the next week.
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Yeah i still don’t. I mix my sub/spawn open air… which is probably the frustration i have with the super slow colonizing of the PE.

When i did horse manure i sterilized it after multiple pasteurized attempts…. The rest of the sub i pasteurized. Then mixed it.

The shit just seemed to contaminate unless I sterilized it separately. Even then i feel a fast colonizer is the absolute key to manure… just something to keep in mind over other traits if you want to avoid grey hair and balding lol
 
Aqua Man said:
Yeah i still don’t. I mix my sub/spawn open air… which is probably the frustration i have with the super slow colonizing of the PE.

When i did horse manure i sterilized it after multiple pasteurized attempts…. The rest of the sub i pasteurized. Then mixed it.

The shit just seemed to contaminate unless I sterilized it separately. Even then i feel a fast colonizer is the absolute key to manure… just something to keep in mind over other traits if you want to avoid grey hair and balding lol
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for sure. i never had problems with horse manure way back when. now the biggest problem i'm having is getting a hold of the shit in the first place. i've also always been pretty diligent about how i pasteurize. always for a minimum of two hours at minimum 140F, preferably closer to 150F, and never passing 160F. 160F is just too hot and does more harm than good. those thermophilic bacteria are super important for offering protection while the mycelium we want takes hold.
 
curiosity got the best of me, popped a corner up for a little peek at both tubs. colonization is fuckin ROCKIN boys. gonna decrease the full cover come off from four days out to Sunday. Probably won't be at full colonization but should be pretty damn close.
 
Aqua Man said:
Moved on to some big tubs. May post pics when they start to fruit.

@Zen_seeker was it you i was going to get some spawn jars to a long time ago?

I finally up and running at a decent level now that i dropped the PE. If so i have jars ready to send
Click to expand...
Cool 👍

PM me what you need.
 
haha so the pink buffalo bags definitely went bacterial. Checked em yesterday and those bags had swollen up like balloons and smelled god awful. so, back at square one for those guys. still have some LC so gonna knock up a couple grain bags and see about getting that back to actually doing something productive.

gonna introduce fruiting conditions for the JMF tubs today. also have a long list of shit to knock out in front of the flow hood. might even work in a PC session on some test tubes for slants. pics will come later.
 
Aqua Man said:
Moved on to some big tubs. May post pics when they start to fruit.

@Zen_seeker was it you i was going to get some spawn jars to a long time ago?

I finally up and running at a decent level now that i dropped the PE. If so i have jars ready to send
Click to expand...
a few of us haha

but ur in canada, eh
 
Zen_seeker said:
I like the grow tent. Best use I’ve seen for these little clear 3-4 shelf models. 👍
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best thing is it cost less to build than buy the same damn thing from places like North Spore. Greenhouse was on sale for like $25, ultrasonic humidifier was $40. Had a spare 6" inline fan and ducting collecting dust, so kinda free, I guess? $10 on lava rocks to line the drip tray. $10 on a small MERV-13 filter. All in less than $100 not including the tubs.

I did modify the humidifier with a vacuum hose for a shop vac so that it sits outside of the greenhouse since get this. humidifiers don't last long in 90%+ humidity lol. if i were to do something like it again, I'd probably spend a bit extra on some real flanges for the humidifier tubing, and the exhaust fan connection.

at this point I'm passively shopping around for grow tents in some uncommon dimensions because they'll be more cost effective and won't require the same kind of modifications to become function for this purpose.
 
the challenge of actually dialing in the martha have begun. condensation is sticking around on the surface of the tubs, and the controller doesn't seem all that accurate. i have a second hygrometer in there and there is a pretty significant discrepancy between the controller and the hygrometer, and with that i think it's safe to say the hygrometer is probably right given the presence of condensation.

dialed back the controller quite a bit and am going to run the exhaust fan non-stop for the next couple hours to see if i can get the condensation to dissipate. not too worried about the tubs drying out but definitely don't want to be seeing any water pooling now that things are starting to knot up.
 
GoodShit97 said:
I wanna grow mushrooms, it's just looks confusing to me lol.
So I just live through yall
Click to expand...
Mycology was my first love, I started growing these years before I started cannabis. It seems overwhelming at first but it really is simple and is low cost when your growing for yourself and the homies.
 
SpectrumFarms said:
Mycology was my first love, I started growing these years before I started cannabis. It seems overwhelming at first but it really is simple and is low cost when your growing for yourself and the homies.
Click to expand...
I'm not a science guy.
Half the stuff you guys say I don't even get lmao 🤣
 
same. i wasn't able to keep a house plant alive before i started growing mushrooms. everything changed after i started growing them. and now, there are so many low effort techniques that one can easily produce personal quantities with a tiny bit of shelf space in a closet and a couple hours of effort.
 
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