All things shrooms

[tobh]

the mycellium bacteria i do have access to test with has organic compounds in it, maybe once it is consumed then maybe i could test feeding it salts?

mycelium != bacteria. two different microorganisms. in our case, we want to avoid bacteria as it is significantly more aggressive than mycelium at early stages and can become quite hazardous.

like, literally make you sick, hospitalize you, or worse. bacteria is bad, mkay.

 

[Aqua Man]

the mycellium bacteria i do have access to test with has organic compounds in it, maybe once it is consumed then maybe i could test feeding it salts?

Mycelium is not bacteria… infact they hate each other and fight to the death. Bacteria produce anti fungal compounds and mycelium produce anti bacterial compounds…. Thus penicillin

 

[Observer]

I believe they only eat organic matter. But i could be wrong. My understanding is you can’t just feed them synthetic nutrients

This would explain why mushroom compost is so popular for plant. If they ate up synthetic or ionic form they would take up all the nutrients that plant do and it wouldn’t be compost. I believe they break down organic matter and inturn supply ionic nutrients to the soil

that makes sense

 

[Observer]

mycelium != bacteria. two different microorganisms. in our case, we want to avoid bacteria as it is significantly more aggressive than mycelium at early stages and can become quite hazardous.

like, literally make you sick, hospitalize you, or worse. bacteria is bad, mkay.

my mistake.

thanks yall for educating me

 

[Observer]

Mycelium is not bacteria… infact they hate each other and fight to the death.

my mistake

Bacteria produce anti fungal compounds and mycelium produce anti bacterial compounds….

thats funny

 

[tobh]

my mistake.

thanks yall for educating me

you're learnin! it's all good man. clandestine mycology is fascinating, and vast. there are a lot of things you'll pick up on fairly quickly.

then, some day when you get around to doing agar, you'll start doing real interesting shit like taking a petri dish, handing it to your kid, and telling them to take the lid off and go run around the yard waving the dish around like a mad person. they'll love it, you'll love it, then get freaked out by all the crazy molds, fungi, and bacterium you collected.

there's an awesome thread on shroomery where people do agar art. it's madness.

 

[Aqua Man]

my mistake

thats funny

You know your fungi is about to become contaminated when you see metabolites being excreted to combate the fungi. Trichoderma are the bane of mushroom mycelium

 

[Aqua Man]

you're learnin! it's all good man. clandestine mycology is fascinating, and vast. there are a lot of things you'll pick up on fairly quickly.

then, some day when you get around to doing agar, you'll start doing real interesting shit like taking a petri dish, handing it to your kid, and telling them to take the lid off and go run around the yard waving the dish around like a mad person. they'll love it, you'll love it, then get freaked out by all the crazy molds, fungi, and bacterium you collected.

there's an awesome thread on shroomery where people do agar art. it's madness.

I loved doing isolations on agar you can distinguish between subspecies and watch the fight… so much can be learned from agar and its so cheap to do

 

[tobh]

Trichoderma are the bane of mushroom mycelium

facts. trich moves like 10x the speed of psilocybe mycelium and it's literally. fucking. everywhere. great for soil grows, terrible for grains though.

 

[Observer]

I lived doing isolations on agar you can distinguish between subspecies and watch the fight… so much can be learned from agar and its so cheap to do

That sounds cool to watch

I gotta get the basics first

 

[Observer]

facts. trich moves like 10x the speed of psilocybe mycelium and it's literally. fucking. everywhere. great for soil grows, terrible for grains though.

Why does psilocybe move so slow?

 

[Observer]

wanted to take this, and grow some mycelium to play around with. Did last year with oatmeal

 

[tobh]

I loved doing isolations on agar you can distinguish between subspecies and watch the fight… so much can be learned from agar and its so cheap to do

after pouring ~50 plates in a SAB i finally bit the bullet and bought a 99.97% HEPA filter (24"x36") and a dayton 1tdu2 blower. still need to get plywood to assemble the thing. but laminar flow + agar is like the money shot for mycology work. pouring plates in a SAB sucks, and my contam rate has averaged around 30%, which really sucks.

this damn house is so full of spores, though, there's not much I can do to avoid it.

 

[tobh]

Why does psilocybe move so slow?

that's the thing, psilocybe is one of the fastest and most aggressive mushroom varieties on earth. trichoderma is a mold (still a type of fungus) and it just moves faster. i've seen jars and bags that were contaminated go from one tiny spec of green to 50% consumed in a matter of 48 hours before. that stuff takes no prisoners man.

 

[Observer]

that's the thing, psilocybe is one of the fastest and most aggressive mushroom varieties on earth. trichoderma is a mold (still a type of fungus) and it just moves faster. i've seen jars and bags that were contaminated go from one tiny spec of green to 50% consumed in a matter of 48 hours before. that stuff takes no prisoners man.

can one grow mycellium from something else and inject cubes'spores into?

 

[Aqua Man]

after pouring ~50 plates in a SAB i finally bit the bullet and bought a 99.97% HEPA filter (24"x36") and a dayton 1tdu2 blower. still need to get plywood to assemble the thing. but laminar flow + agar is like the money shot for mycology work. pouring plates in a SAB sucks, and my contam rate has averaged around 30%, which really sucks.

this damn house is so full of spores, though, there's not much I can do to avoid it.

Thats what i did built a hepa positive pressure box

 

[Aqua Man]

can one grow mycellium from something else and inject cubes'spores into?

They will fight

 

[Observer]

i dont understand mycellium i guess, theres different kinds of mycellium or all fungi grow a mycellium

 

[Observer]

They will fight

lol tiny microbes with tiny boxing gloves comes to mind

 

[2Bad]

after pouring ~50 plates in a SAB i finally bit the bullet and bought a 99.97% HEPA filter (24"x36") and a dayton 1tdu2 blower. still need to get plywood to assemble the thing. but laminar flow + agar is like the money shot for mycology work. pouring plates in a SAB sucks, and my contam rate has averaged around 30%, which really sucks.

this damn house is so full of spores, though, there's not much I can do to avoid it.

That's crazy! I did pretty well with mine . I also used like disinfectant spray and sprayed the room like 20 mins before I woked.

 

[Observer]

according to ChatGPT3

"""Psilocybin cubensis can be cultivated using both natural and synthetic nutrient sources. While many growers prefer using organic materials like grains and natural supplements, it is possible to provide synthetic nutrients to Psilocybin cubensis as well."""

obviously im more inclined to believe you guys of course, but now i want to experiment.

For Science, Facts and Logic.

 

[Aqua Man]

i dont understand mycellium i guess, theres different kinds of mycellium or all fungi grow a mycellium

All mycelium are fungi but not all fungi produce mycelium… it’s basically a stage of fungal growth

 

[Observer]

you're learnin! it's all good man. clandestine mycology is fascinating, and vast. there are a lot of things you'll pick up on fairly quickly.

then, some day when you get around to doing agar, you'll start doing real interesting shit like taking a petri dish, handing it to your kid, and telling them to take the lid off and go run around the yard waving the dish around like a mad person. they'll love it, you'll love it, then get freaked out by all the crazy molds, fungi, and bacterium you collected.

there's an awesome thread on shroomery where people do agar art. it's madness.

i learn quick! just need someone to work with me

 

[Observer]

All mycelium are fungi but not all fungi produce mycelium… it’s basically a stage of fungal growth

thanks

 

[Observer]

mycelium != bacteria. two different microorganisms. in our case, we want to avoid bacteria as it is significantly more aggressive than mycelium at early stages and can become quite hazardous.

like, literally make you sick, hospitalize you, or worse. bacteria is bad, mkay.

i can see my self growing some mushrooms but contaminated and fuckin my self up lol

or would they even fruit if it was contam

 

[Observer]

Send help, little human keeps following me around.

Haha

 

[tobh]

That's crazy! I did pretty well with mine . I also used like disinfectant spray and sprayed the room like 20 mins before I woked.

yeah, it got irritating as hell tbh. aseptic technique is on point, i never had these kind of problems when i lived in NM. being the pnw is a whole other beast though. only place i've had contam rates like i have, and also the only place i've had multiple different kinds of plants fail due to botrytis.

shutting off the HVAC at least three hours before doing the work, bombing the working room with ozium multiple times before starting work, cleaning everything with 70% ISO... showering, wearing gloves, arm covers, and a respirator... none of it matters here apparently. the new house will be getting a dedicated clean room for the flow hood. a buddy and i are working on building a mushroom farm, he's focusing on the commercial varieties (oysters, buttons, shitake, lion's mane, etc) and i'm focusing on the exotics (reishi, maitake, cordyceps, etc).

so suffice to say i'm done playing fuck around with these high contam rates. gotta get those numbers down.

All mycelium are fungi but not all fungi produce mycelium… it’s basically a stage of fungal growth

this

 

[tobh]

i can see my self growing some mushrooms but contaminated and fuckin my self up lol

or would they even fruit if it was contam

they'll fruit, depending when the contamination hits. on my larger monotubs, i'd typically get three or four flushes before the mean green showed its face. bacterial infections can do some wild shit to the fruit bodies too. you shouldn't eat anything that's been subject to contam, though, calling back to the sick, hospital, dead thing.

you don't want to unalive yourself because of being stubborn and not wanting to toss a failed experiment

Send help, little human keeps following me around.

Haha

ah man... i miss when my son was that age. he's four now, so still a bit of a shadow, but the lack of motor skill mastery at that stage is hilarious

 

[Observer]

they'll fruit, depending when the contamination hits. on my larger monotubs, i'd typically get three or four flushes before the mean green showed its face. bacterial infections can do some wild shit to the fruit bodies too. you shouldn't eat anything that's been subject to contam, though, calling back to the sick, hospital, dead thing.

you don't want to unalive yourself because of being stubborn and not wanting to toss a failed experiment

yea i dont want to be stubborn, just cheap and efficient lol.
gotta be around for her

ah man... i miss when my son was that age. he's four now, so still a bit of a shadow, but the lack of motor skill mastery at that stage is hilarious

its hilarious as she tries to balance and catch her self lol.

i never wanted kids, i never seen my self having a family.

but shes here, i love her and i wouldnt give her up for a $trillion, and im glad shes here.

 

[Observer]

they'll fruit, depending when the contamination hits. on my larger monotubs, i'd typically get three or four flushes before the mean green showed its face. bacterial infections can do some wild shit to the fruit bodies too. you shouldn't eat anything that's been subject to contam, though, calling back to the sick, hospital, dead thing.

you don't want to unalive yourself because of being stubborn and not wanting to toss a failed experiment

ah man... i miss when my son was that age. he's four now, so still a bit of a shadow, but the lack of motor skill mastery at that stage is hilarious

so if youre fruit is contam its noticeable though? on the mushroom?

 

[tobh]

so if youre fruit is contam its noticeable though? on the mushroom?

not always. in the more advanced bacterial infections i've seen firsthand, the fruit bodies will mutate pretty severely and be anemic vs healthy fruits.

yea i dont want to be stubborn, just cheap and efficient lol.
gotta be around for her

its hilarious as she tries to balance and catch her self lol.

i never wanted kids, i never seen my self having a family.

but shes here, i love her and i wouldnt give her up for a $trillion, and im glad shes here.

same. tequila made my son, and he's the best damn thing to happen to me. now if only the mental health and vices would catch up with that positivity, it'd be a pretty great life.

 

[Observer]

yeah, it got irritating as hell tbh. aseptic technique is on point, i never had these kind of problems when i lived in NM. being the pnw is a whole other beast though. only place i've had contam rates like i have, and also the only place i've had multiple different kinds of plants fail due to botrytis.

shutting off the HVAC at least three hours before doing the work, bombing the working room with ozium multiple times before starting work, cleaning everything with 70% ISO... showering, wearing gloves, arm covers, and a respirator... none of it matters here apparently. the new house will be getting a dedicated clean room for the flow hood. a buddy and i are working on building a mushroom farm, he's focusing on the commercial varieties (oysters, buttons, shitake, lion's mane, etc) and i'm focusing on the exotics (reishi, maitake, cordyceps, etc).

so suffice to say i'm done playing fuck around with these high contam rates. gotta get those numbers down.

this

damn all that and still high contam rate?

 

[Observer]

not always. in the more advanced bacterial infections i've seen firsthand, the fruit bodies will mutate pretty severely and be anemic vs healthy fruits.

ahh ok

same. tequila made my son, and he's the best damn thing to happen to me. now if only the mental health and vices would catch up with that positivity, it'd be a pretty great life.

yea..liquor & coke.

indubitably lol

 

[tobh]

damn all that and still high contam rate?

yeah. it's all good though. challenges force you to improve. you either persevere, or give up, and i'm not a quitter

 

[Aqua Man]

Send help, little human keeps following me around.

Haha

The ruler of your heart for the rest of your life… even though when they are teenagers the develop into some sort of spawn mutation that allows us the ability to be thankful when they move out… then ita back to ruling your heart again… oh and the worrying will shorten your lifespan lol

 

[Aqua Man]

Ince you see contam once i found it very easy to spot

 

[Observer]

The ruler of your heart for the rest of your life… even though when they are teenagers the develop into some sort of spawn mutation that allows us the ability to be thankful when they move out… then ita back to ruling your heart again… oh and the worrying will shorten your lifespan lol

So true lol.

 

[Observer]

yeah. it's all good though. challenges force you to improve. you either persevere, or give up, and

i'm not a quitter

Damn right we aren't

 

[Observer]

Thanks y'all Lot for you're insights.

 

[Observer]

Expected Delivery by​

Thursday 25 May 2023 by​

9:00pm ​